The revolutionary 4D Organ-on-chip platform
NEXT GENERATION IN-VITRO TESTING WITH TISSUE-ON-CHIP AND MICROFLUIDING CHIP IN ONE PLATFORM:
Prior to market launch of personal care products and the clinical trials of therapeutics, stakeholders within the chemical, ingredient, FMCG, and pharmaceutical industries need to assess and determine their safety, absorption and efficacy. Traditionally, these evaluations have been conducted in vivo on animal models.
Over the last few years, several advances have been made toward the development and production of in vitro human skin models for the analysis and testing of cosmetic and pharmaceutical products. However, these skin models are cultured under static conditions that make them unable to accurately represent normal human physiology. Recent interest has focused on the generation of in vitro 3D vascularized skin models with dynamic perfusion and microfluidic devices known as skin-on-a-chip. These platforms have been widely described in the literature as good candidates for tissue modeling, as they enable a more physiological transport of nutrients and permit a high-throughput and less expensive evaluation of drug candidates in terms of toxicity, efficacy, and delivery.
REVIVO Biosystems’ organ-on-a-chip platforms drastically increase human relevance and productivity and enables future targeted developments for the chemical, personal care and pharmaceutical industries, without animal cruelty.
REVIVO BioSystems has developed an organ-on-achip platform (ReleGO™) for the realistic and time/cost-optimised assessment of ingredients, formulations, and finished products. Such platform combines the principles of miniaturisation and microfluidics with those of biological transport phenomena and tissue engineering. This integration provides the most realistic human barrier tissue models achievable in lab conditions.
REVIVO BioSystems is thus offering a highly representative, automated testing platform that is accurate, efficient, and cost effective. The company is passionate about making significant contributions to a cruelty-free research environment, from reducing and even eliminating animal tests, all the way down to removing animal-derived components in its own tissue cultivation processes.
Innovative in microfluidic technology & Skin-on-a-chip
REVIVO BioSystems is expert in realistic 4D human tissue testing models that include the mimicking of the function of the blood flow, and take human error out of the sampling.
ReleGO™
FULLY AUTOMATED & PROGRAMMABLE
FRACTION COLLECTOR
REVex™
SKIN-ON-CHIP
COMPATIBLE WITH HUMAN EXPLANTS, COMMERCIAL RECONSTRUCTED SKIN SIMULANTS, SYNTHETIC MEMBRANES AND OTHER TISSUES
HOW THE PLATFORM (ReleGO™ & REVex™) WORKS?
Tissue explants (sourced directly by REVIVO BioSystems or obtained from authorized suppliers) and tissue engineered models are loaded and grown, respectively, into the microfluidic chips. A continuous flow of nutrients and reagents through the microfluidic channels mimics the function of the blood flow, thus providing 4D tissue models. This creates a unique micro-environment that establishes natural tissue properties. Continuous flow further enables the long term maintenance of the human tissues and hence the potential to study the biological response over a few weeks (instead of a few days). The flow rate can be fine-tuned and the collection of the outflow is performed automatically at custom-defined time intervals in 96-well collection plates using ReleGO.
This enables the end user to have a kinetic analysis with a 10-fold reduction of man-hours otherwise spent in tedious manual operations. Both topical and systemic product dosages can be studied, with a realistic physiological response. Direct access to the tissues’ external surface enables the topical applications of not only solids, semisolids, and liquids, but also gases and aerosols. Drugs or test compounds can also be delivered via the microfluidic channels, thus mimicking a systemic exposure. Measurement of transepidermal electrical resistance is also enabled, a parameter frequently used to assess the barrier integrity of epithelial tissues.
ReleGO™
FULLY AUTOMATED & PROGRAMMABLE FRACTION COLLECTOR
ReleGO™ is an automated testing system including the functions of fraction collection, temperature control, and enabled for electrode/sensor integrations, in which the microfluidic devices (REVex™) are loaded.
ReleGO™ is not only able to yield more accurate results with convenient automated sampling, but also multi-functional for safety, permeation and efficacy studies of active ingredients and product formulations.
- Up to 12 human skin biopsies, synthetic membranes or 2D/3D organotypic cultures can be loaded in REVex™ chips and run simultaneously.
- ReleGO™ testing system mimics the function of the blood flow and creates In vivo-like conditions.
- Skin-on-chip devices are mounted on ReleGO™ and the eluates collected in 96-well plates.
- ReleGO™ is an automated fraction collector with additional functions of temperature control and fluidic handling.
REVex™
REVex™
SKIN-ON-CHIP
COMPATIBLE WITH HUMAN EXPLANTS, COMMERCIAL RECONSTRUCTED SKIN SIMULANTS, SYNTHETIC MEMBRANES AND OTHER TISSUES
APPLICATION
Looking for a guideline for your own application with ReleGo microfluidic system?
FEATURES
INCREASED SENSITIVITY ON ReleGO™
ENHANCED PRECISION ON REVex™
MORE SAMPLES OUT OF 1 BIOPSY - LESS BIOMATERIAL USED WITH REVEX™
UNIQUE SYSTEMIC EFFECT SIMULATION
10 X SAVINGS MANPOWER & BIOMATERIAL
Comparison of ReleGO system with other methods
Source Multi-chamber microfluidic platform for highprecision skin permeation testing
M. Alberti, †a Y. Dancik, †*bc G. Sriram, b B. Wu,a Y. L. Teo,c Z. Feng,a
M. Bigliardi-Qi,bc R. G. Wu,a Z. P. Wang‡*a and P. L. Bigliardi‡bc
| Rele GO TM Platform | Automated sampler apparatus for vertical diffusion cells | Automated sampler apparatus for flow-through cells | Maual Vertical Franz Diffusion cell Apparatus | |
| Mimic natural conditions | ✔ | ✖ | ✖ | ✖ |
| Cost factor | 1 | 5 | 2 | 1-2 |
| Dimensions | 1 | 6 | 3 | 3 |
| Weight | 1 | 5-6 | 3-4 | 3 |
| Automated sampling | ✔ | ✔ | ✔ | ✖ |
| Samples per run | 12 | 12 | 7 | 9 |
| Size of skin specimen per replicate | 0.5 cm | 2-4 cm | 2-4 cm | 2-4 cm |
| Manhours for a 24h study | 1h | 9h | 9h | >18h |
| Long-term maintenance of tissue models | ✔ | ✖ | ✖ | ✖ |
| Sampled columns are collected in | 96-well plates | Cuvettes | Customized cuvettes | Cuvettes |
| Automated cleaning | ✔ | ✖ | ✖ | ✖ |
FREQUENTLY ASKED QUESTIONS
A: Due to Limitations on 2D cultures and 3D organ models, other new technologies such as microfabrication for biological purposes have been created. This approach directed to the development of the so-called organs-on-chips. These are microfluidic devices with micron size chambers that allow the dynamic culture of cells inside, in order to simulate or mimic the physiology of a tissue or organ. It is possible to apply various physical or chemical stimuli on the tissue inside the chip and simulate the body environment.
The possibility of applying different physical or chemical stimuli to the tissue inside the chip might help with the recreation of its physiology in a more accurate manner than that of a static and traditional 3D culture, having a better control of the cell microenvironment.
A: It’s a novel model to drive productivity up and cost down, while more importantly moving away from cruel and animal-based testing and working with ex-vivo human tissue explants or in vitro tissue engineered models.. ReleGO™ is fully automated and easy-to-use platform, mimicking in-vivo-like conditions for your safety, efficacy, and permeation studies.
A: The minimum and maximum allowable flow rates of the ReleGO™ system are 0.5 to 120 uL/min. However, in practice for ex vivo and in vitro studies, a flow rate of 1-10 uL/min is more commonly used. As an example, we’d recommend a flow rate of 4 uL/min for permeation studies on the ReleGO™.You will also be delighted to know that ReleGO™ has been programmed to alert users if too high a flow rate is being selected for your chosen experimental duration, time intervals, and type of collection plate. With this, users are free to explore various flow rate settings to customise a 4D ex vivo and in vitro experimental design.
A: The experimental duration can vary based on multiple factors such as the type of tissue used, experimental setup, and the particular research inquiry being pursued. On ReleGO, you can easily program the test period to range from several hours to an extended continuous duration of 58 days!
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